A cAMP-dependent phosphorylated motility protein in bovine epididymal sperm.
نویسندگان
چکیده
منابع مشابه
Protein phosphatase PP1γ2 in sperm morphogenesis and epididymal initiation of sperm motility
The serine/threonine phosphatase (PP1) isoform PP1γ2, predominantly expressed in the testis, is a key enzyme in spermatozoa. High PP1γ2 catalytic activity holds motility in check in immature spermatozoa. Inhibition of PP1γ2 causes motility initiation in immature spermatozoa and motility stimulation and changes in flagellar beat parameters in mature spermatozoa. The PP1γ2 isoform is present in a...
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Using a selective inhibitor of cAMP-dependent protein kinase, N-[2(methylamino)ethyl]-5-isoquinolinesulfonamide (H-8), the requirement for cAMP-dependent phosphoproteins in the initiation of dog sperm flagellar motility was examined. H-8 inhibited motility of live as well as reactivated sperm in a dose-dependent manner. The half-maximal inhibition of reactivated motility (32 microM) paralleled ...
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Background: There is a general deterioration in sperm quality depending on time postmortem. Refrigeration of the epididymes down to around 5°C is the best strategy to lower this damage and there are many dissim ilarities between species, possibly due to differences on cold shock endurance of epididymal sperm. Our objective was to determine the effect of 5°C and 37°C on goat epididymal sperms mo...
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In the present study, the fertilizing potential of semen recovered from slaughtered bulls epididymis was evaluated after cryopreservation, by conventional techniques and flow cytometry methods. The cauda epididymal was dissected and sperm were recovered and evaluated for volume, sperm concentration, and membrane and acrosome integrity using a flow cytometer. Sperm fertility potential was tested...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1980
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)86129-x